A complete spinal cord injury results, tragically, in total paralysis of all limbs and muscles below the injury site. But now, scientists at EPFL have demonstrated in mice a new gene therapy that can regenerate nerves and restore the ability to walk.
The spinal cord is, to use an outdated tech term, the information super-highway of the body. Messages between the brain and every other part of the body travel up the thick bundle of nerves there at incredibly fast speeds. As such, damage to this conduit can be debilitating, leaving patients with no sensation or mobility in affected areas.
Unsurprisingly, finding new ways to repair these injuries is a key area of research, with recent studies finding some success using implants that bypass the injured area, nerve cell transplants, and proteins, molecules or compounds that help stimulate nerve regrowth. The EPFL team had previously managed to regenerate nerve fibers using gene therapy, but had limited success.
“Five years ago, we demonstrated that nerve fibers can be regenerated across anatomically complete spinal cord injuries,” said Mark Anderson, senior author of the study. “But we also realized this wasn’t enough to restore motor function, as the new fibers failed to connect to the right places on the other side of the lesion.”
To fix the issue, the researchers studied the natural repair processes that take place after a partial spinal cord injury. Using a technique called single-cell nuclear RNA sequencing, the team identified the specific axons that need to be repaired to restore motor function, and how they can find the right target on the other side of the injury.
From this analysis, the researchers developed a new gene therapy that works in a few ways at once to boost the reconnection of nerves. The therapy activates growth programs in certain neurons to regenerate the key nerve fibers, upregulates certain proteins that help the neurons grow through the damaged tissue, and added molecules that guide those regenerating nerves to their targets on the other side.
In tests in mice with complete spinal cord injuries, the team found that the treated animals regained the ability to walk in a matter of months, ending up with a gait similar to those of mice that had recovered from a partial injury.
While there’s still plenty more work to be done before this kind of therapy could be applied to humans, the team says that it marks a key step towards that eventual goal.
“We expect that our gene therapy will act synergistically with our other procedures involving electrical stimulation of the spinal cord,” said Grégoire Courtine, senior author of the study. “We believe a complete solution for treating spinal cord injury will require both approaches – gene therapy to regrow relevant nerve fibers, and spinal stimulation to maximize the ability of both these fibers and the spinal cord below the injury to produce movement.”
The research was published in the journal Science.
Thursday, October 12, 2023
Gene Therapy for spinal damage
Thursday, August 18, 2022
The resurrection of the Tasmanian Tiger
From The Guardian
Scientists in Australia and the US have launched an ambitious multimillion-dollar project to bring back the thylacine, a marsupial that died out in the 1930s, and reintroduce it to its native Tasmania.
The thylacine, also known as the Tasmanian tiger, is the second undertaking by Colossal, a Texas-based biotechnology “de-extinction” company that last year announced it planned to use genetic engineering techniques to recreate the woolly mammoth and return it to the Arctic tundra.
Its new project is a partnership with the University of Melbourne, which earlier this year received a $5m philanthropic gift to open a thylacine genetic restoration lab. The lab’s team has previously sequenced the genome of a juvenile specimen held by Museums Victoria, providing what its leader, Prof Andrew Pask, called “a complete blueprint on how to essentially build a thylacine”.
The thylacine was Australia’s only marsupial apex predator. It once lived across the continent, but was restricted to Tasmania about 3,000 years ago. Dog-like in appearance and with stripes across its back, it was extensively hunted after European colonisation. The last known survivor died in captivity in 1936. Despite many reported sightings in the decades that followed, and some quixotic attempts to prove its ongoing existence, it was officially declared extinct in the 1980s.
The scientists aim to reverse this by taking stem cells from a living species with similar DNA, the fat-tailed dunnart, and turning them into “thylacine” cells – or the closest approximation possible – using gene editing expertise developed by George Church, a professor of genetics at Harvard Medical School and Colossal’s co-founder. New marsupial-specific assisted reproductive technologies will be needed to use the stem cells to make an embryo, which would be transferred into either an artificial womb or a dunnart surrogate to gestate.
Pask said the partnership was the most significant contribution ever made to marsupial conservation in Australia as more than 30 scientists worked to accelerate the “massive grand challenge” of bringing the thylacine back from the dead. He believed the first joeys could be born in 10 years.
Colossal’s chief executive and other co-founder, the tech and software entrepreneur Ben Lamm, was more bullish, believing it was possible in less than six years, the timeframe that the company had set itself to produce the first set of mammoth calves.
“I think it’s highly probable this could be the first animal we de-extinct,” Lamm told the Guardian.
The challenges faced by the project are significant, and the scientists acknowledge several breakthrough steps will have to land for it to succeed. On reproductive technology, Pask said: “We are pursuing growing marsupials from conception to birth in a test-tube without a surrogate, which is conceivable given infant marsupials’ short gestation period and their small size.”
If successful, the plan would be to introduce the animal in a controlled setting on Tasmanian private land with an eventual goal of returning it to the wild. Pask said the researchers said they saw the work as not just bringing back long-dead species, but helping develop technology that could be applied to addressing today’s global extinction crisis.
Friday, March 4, 2022
20 Things which will change the world by 2040
1. CHEAP ELECTRICITY
Wind costs are falling by 5-10% per annum, solar by 10% to 20% and batteries by 15% plus. Wind's cost declines will prolly slow over the next 10 years—it's a mature technology. But the cost declines in solar are likely to continue, and in batteries, there's a real chance they'll accelerate. If these trends continue (and why won't they?), in 10 years electricity will cost 25% of what it does now. If the trend decline then halves to, say 7% a year, then in 20 years, electricity will cost just 10% of what it does now. Cheap energy supercharges economic growth. The low oil price from 1945 to 1973 helped drive rapid and sustained growth in the world economy. Cheap renewables will do the same over the next 20 years and beyond.
2. EVS
They're going to be cheaper than ICEVs (internal combustion vehicles) to buy, and much cheaper to run. They'll be replacing ICEVs so the shifts in society might not seem dramatic. But with AIs running them, transport as a service will become common. You'll summon a car using your phone, and it will drive itself to where you are and then to where you want to go. Because EVs will last much longer than ICEVs and will be significantly cheaper to run, "transport as a service" will be a popular way for people to get around. Car sales are likely to decline by 50% plus, as TAAS takes off. Air pollution in cities will end. By 2040, most of the world's vehicle fleet will be electric. Maybe hydrogen fuel-cell, but I doubt it. The cost of building a hydrogen refuelling network will be much more costly than just attaching your car to an already existing network, the electric grid. And the energy efficiency of the hydrogen cycle is much lower than batteries.
3. AI
I don't think we'll have true AI, as in sentient robots. But we will have very sophisticated computerised control systems, such as those which will allow for self-driving cars and self-landing rockets. This has been made possible by the 5 or 6 orders of magnitude decline in the costs of and size of super computers, as Tony Seba points out. SpaceX's ability to land and re-use its rockets would not have been possible without the advances in computing power. These advances and changes all interact.
4. 3-D PRINTING
This cuts the cost of manufacturing metal things by at least half, because there's much less scrap. It also reduces the stock of parts you have to keep on hand. And allows you to make more complicated things, like SpaceX's extraordinary new Raptor rocket engines. On the ISS, there is a 3-D printer to make spare parts. On Mars, and the Moon, 3-D printers will be used to build habitats; to make things which would take too long or are too expensive to get from Earth; and to make things which have short production runs or are experimental.
5. VAT MEAT, MILK AND FISH
Cheap energy will change agriculture. Right now, 20% of Australia's tomatoes come from a factory in the semi-desert in the north of the State of South Australia, using desalinated sea water and growing the tomatoes in greenhouses. This undertaking uses no fossil fuel at all. Animal rearing uses vast areas of land, is highly polluting, and contributes 20% to global CO₂ emissions. Vat meats and fish are already starting to take off. Their costs are declining year by year. By 2040, they will become the norm.
6. CHEAP ACCESS TO SPACE
Cheap access to space will change everything. To settle Mars, we'll need to rapidly improve a whole range of technologies, like vat meat production, genuine air conditioning (meaning far more than just heating and cooling), hydroponics, water purification, extracting CO₂ from the atmosphere, genetic modification, medicine, and so on.
7. ASTEROID MINING
With cheap access to space also comes asteroid mining. Because the asteroids aren't in deep gravity wells like the Earth or Mars, nudging them into orbits which intersect Earth's or Mars's will be cheap. The resources of a single medium-sized asteroid, for example for rare earth metals, will more than equal all the rare earth metals that have ever been mined on Earth. We will prolly stop stripping the Earth to produce metals and minerals and instead start disassembling asteroids to do that. The world's major resource companies will be asteroid miners.
But some of these will be used in space manufacturing. Why take stuff into the gravity well when you can build it in LEO? Asteroid mining will be even more important on Mars, as asteroids will likely provide the volatiles needed to give Mars an atmosphere dense enough for humans to work in without needing to wear pressurised space suits.
8. BECOME A MULTI-PLANETARY SPECIES
Our first colony will be Mars. Read the Red Mars trilogy to see how colonising Mars will change Earth too. Not just in technological advances but also in social advances. Looking down on Mars and Earth from space will change mankind's perception of itself. As Robert Zubrin says, knowing that there is no shortage of resources because we have unlimited resources in space means that most of the causes of war on Earth will disappear. Of course, no matter how technologically advanced and prosperous humans become, there is no reason to suppose we will ever be more intelligent, less venal, less greedy, and less petty.
After we colonise Mars, we'll start on Venus. That'll be much harder. But by then we will also have colonies in the asteroid belt and large inhabited space stations in orbit round the Earth and Mars. We will truly be a multi-planetary species. And that will change everything.
9. TRULY GLOBAL HIGH-SPEED INTERNET
10. TERRAFORMING THE EARTH
11. GENE THERAPY & GENETIC MODIFICATION
12. THE RISE OF AFRICA
13. THE END OF NEO-LIBERALISM
14. A UNIVERSAL BASIC INCOME
15. HYPERSONIC INTERCONTINENTAL FLIGHTS
16. NEURALINK
Tuesday, September 14, 2021
Bringing back the woolly mammoth
From Melbourne's The Age newspaper
Ten thousand years after woolly mammoths vanished from the face of the Earth, scientists are embarking on an ambitious project to bring the beasts back to the Arctic tundra.
The prospect of recreating mammoths and returning them to the wild has been discussed – seriously at times – for more than a decade, but on Monday researchers announced fresh funding they believe could make their dream a reality.
The boost comes in the form of $15m (£11m) raised by the bioscience and genetics company Colossal, co-founded by Ben Lamm, a tech and software entrepreneur, and George Church, a professor of genetics at Harvard Medical School who has pioneered new approaches to gene editing.
The scientists have set their initial sights on creating an elephant-mammoth hybrid by making embryos in the laboratory that carry mammoth DNA. The starting point for the project involves taking skin cells from Asian elephants, which are threatened with extinction, and reprogramming them into more versatile stem cells that carry mammoth DNA. The particular genes that are responsible for mammoth hair, insulating fat layers and other cold climate adaptions are identified by comparing mammoth genomes extracted from animals recovered from the permafrost with those from the related Asian elephants.
These embryos would then be carried to term in a surrogate mother or potentially in an artificial womb. If all goes to plan – and the hurdles are far from trivial – the researchers hope to have their first set of calves in six years.
“Our goal is to make a cold-resistant elephant, but it is going to look and behave like a mammoth. Not because we are trying to trick anybody, but because we want something that is functionally equivalent to the mammoth, that will enjoy its time at -40C, and do all the things that elephants and mammoths do, in particular knocking down trees,” Church told the Guardian.
The project is framed as an effort to help conserve Asian elephants by equipping them with traits that allow them to thrive in vast stretches of the Arctic known as the mammoth steppe. But the scientists also believe introducing herds of elephant-mammoth hybrids to the Arctic tundra may help restore the degraded habitat and combat some of the impacts of the climate crisis. For example, by knocking down trees, the beasts might help to restore the former Arctic grasslands.
Not all scientists suspect that creating mammoth-like animals in the lab is the most effective way to restore the tundra. “My personal thinking is that the justifications given – the idea that you could geoengineer the Arctic environment using a heard of mammoths – isn’t plausible,” said Dr Victoria Herridge, an evolutionary biologist at the Natural History Museum.
“The scale at which you’d have to do this experiment is enormous. You are talking about hundreds of thousands of mammoths which each take 22 months to gestate and 30 years to grow to maturity.”
Lamm said: “Our goal isn’t just to bring back the mammoth, but to bring back interbreedable herds that are successfully rewilded back into the Arctic region.”
Whether Asian elephants would want to breed with the hybrids is, for now, unknown. “We might have to give them a little shave,” said Church.
Gareth Phoenix, a professor of plant and global change ecology at the University of Sheffield, said: “While we do need a multitude of different approaches to stop climate change, we also need to initiate solutions responsibly to avoid unintended damaging consequences. That’s a huge challenge in the vast Arctic where you have different ecosystems existing under different environmental conditions.
“For instance, mammoths are proposed as a solution to help stop permafrost thaw because they will remove trees, trample and compact the ground and convert landscapes to grassland, which can help keep the ground cool. However, we know in the forested Arctic regions that trees and moss cover can be critical in protecting permafrost, so removing the trees and trampling the moss would be the last thing you’d want to do.”
What a piece of work is a man! How noble in reason! how infinite in faculty! in form, in moving, how express and admirable! in action how like an angel! in apprehension how like a god! the beauty of the world! the paragon of animals! And yet, to me, what is this quintessence of dust?


